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PF ameliorated HFFD-induced hepatic fibrosis progression in MASLD mice. (A and B) Representative images and quantification of hepatic Sirius scarlet staining. ( n = 3, scale bar = 50 μm). (C to E) Serum fibrosis factor HA, LN, and Col-IV levels. (F) Liver mRNA levels of α-SMA, Timp1, Tgf-β1, Col1a1, Fn1 , Pdgfrβ , and PAI-1 . (G and H) Western blot of α-SMA, TIMP1, and TGF-β1 protein expression and their quantification. Data were presented as the mean ± SEM ( n = 6). # P < 0.05, ## P < 0.01, ### P < 0.001 versus the ND group; * P < 0.05, ** P < 0.01, *** P < 0.001 versus the HFFD group.

Journal: Research

Article Title: Paeoniflorin Ameliorates Metabolic Dysfunction-Associated Steatotic Liver Disease by SYK/SH3BP2 Signaling Pathway

doi: 10.34133/research.1100

Figure Lengend Snippet: PF ameliorated HFFD-induced hepatic fibrosis progression in MASLD mice. (A and B) Representative images and quantification of hepatic Sirius scarlet staining. ( n = 3, scale bar = 50 μm). (C to E) Serum fibrosis factor HA, LN, and Col-IV levels. (F) Liver mRNA levels of α-SMA, Timp1, Tgf-β1, Col1a1, Fn1 , Pdgfrβ , and PAI-1 . (G and H) Western blot of α-SMA, TIMP1, and TGF-β1 protein expression and their quantification. Data were presented as the mean ± SEM ( n = 6). # P < 0.05, ## P < 0.01, ### P < 0.001 versus the ND group; * P < 0.05, ** P < 0.01, *** P < 0.001 versus the HFFD group.

Article Snippet: LX2 cells or transfected LX2 cells were treated with 10 ng/ml TGF-β1 (HY-P7118, MCE) for 24 h [ ].

Techniques: Staining, Western Blot, Expressing

PF alleviated MASLD fibrosis progression in vitro by inhibiting the SYK/SH3BP2 pathway. (A) LX2 cell survival ( n = 3). (B to G) Western blot of P-SYK, SYK, SH3BP2, α-SMA, TIMP1, and Tgf-β1 protein expression and their quantification. (H) mRNA levels of SYK, SH3BP2, α-SMA, TIMP1, Tgf-β1, Col1a1, FN1, Pdgfrβ , and PAI-1 . Data were presented as the mean ± SEM ( n = 4). # P < 0.05, ## P < 0.01, ### P < 0.001 versus the Tgf-β1 group; * P < 0.05, ** P < 0.01, *** P < 0.001 versus the Tgf-β1+oe-SYK group.

Journal: Research

Article Title: Paeoniflorin Ameliorates Metabolic Dysfunction-Associated Steatotic Liver Disease by SYK/SH3BP2 Signaling Pathway

doi: 10.34133/research.1100

Figure Lengend Snippet: PF alleviated MASLD fibrosis progression in vitro by inhibiting the SYK/SH3BP2 pathway. (A) LX2 cell survival ( n = 3). (B to G) Western blot of P-SYK, SYK, SH3BP2, α-SMA, TIMP1, and Tgf-β1 protein expression and their quantification. (H) mRNA levels of SYK, SH3BP2, α-SMA, TIMP1, Tgf-β1, Col1a1, FN1, Pdgfrβ , and PAI-1 . Data were presented as the mean ± SEM ( n = 4). # P < 0.05, ## P < 0.01, ### P < 0.001 versus the Tgf-β1 group; * P < 0.05, ** P < 0.01, *** P < 0.001 versus the Tgf-β1+oe-SYK group.

Article Snippet: LX2 cells or transfected LX2 cells were treated with 10 ng/ml TGF-β1 (HY-P7118, MCE) for 24 h [ ].

Techniques: In Vitro, Western Blot, Expressing